Table 4. Analysis of Technology Options for Affinity Reagent Production

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Table 4. Analysis of Technology Options for Affinity Reagent Production (acronyms)

	Phage Display	Yeast Display	Ribosome and Puromycin Display	DNA or RNA Aptamers	Animals
Strengths	Good diversity Fusion proteins	Liquid and fluorescence-based screening Affinity maturation Fusion proteins	Good diversity Fusion proteins	Good diversity	Many secondary antibodies available
Weaknesses	Slower screening Plate based	Fluorescent tags required that may complicate recognition Reduced cys on targets problematic	Slower screening	Fewer secondary affinity labels Not protein based, so no fusion proteins	Expensive Not high throughput Nonrenewable unless use mAb Slow
Development Targets and Needs	High throughput demonstrated Improved screening	High throughput Improved screening Secondary antibodies that must be developed	Optimization of scaffolds, screening methods, and automation	Optimization of screening methods	Optimization of screen methodologies DNA immunization and improvements in hybridoma production

Phage Display

Yeast Display

Ribosome and Puromycin Display

DNA or RNA Aptamers

Animals

Strengths

Good diversity

Fusion proteins

Liquid and fluorescence-based screening

Affinity maturation

Fusion proteins

Good diversity

Fusion proteins

Good diversity

Many secondary antibodies available

Weaknesses

Slower screening

Plate based

Fluorescent tags required that may complicate recognition

Reduced cys on targets problematic

Slower screening

Fewer secondary affinity labels

Not protein based, so no fusion proteins

Expensive

Not high throughput

Nonrenewable unless use mAb

Slow

Development Targets and Needs

High throughput demonstrated

Improved screening

High throughput

Improved screening

Secondary antibodies that must be developed

Optimization of scaffolds, screening methods, and automation

Optimization of screening methods

Optimization of screen methodologies

DNA immunization and improvements in hybridoma production

June 14-16, 2004, GTL Technology Deep Dive Workshop, Working Group on Genome-Based Reagents

The table above compares and contrasts strengths, weaknesses, and development needs of technologies for use in a high-throughput production environment.

U.S. Department of Energy Office of Science

Genomics:GTL

Table 4. Analysis of Technology Options for Affinity Reagent Production (acronyms)

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